棉花遗传多样性分析SSR标记的快速预筛   总被引：1，自引：0，他引：1  

姜辉  王永翠  高明伟  王秀丽  陈莹  王家宝  柴启超  张超  赵军胜 《中国棉花》2019,46(4):13-16

选用60个棉花基因组SSR标记，40份棉花种质为材料，采用改良DNA混合池的方法，探讨了多态性较好的SSR标记的快速筛选及其在棉花种质遗传多样性分析中的应用效果。结果显示：通过此方法，可以较为快捷地筛选出多态性好的SSR标记。聚类分析表明：筛选出的多态性较好的标记，其聚类分析效果较好，可更好地展现种质资源之间遗传背景的差异。  相似文献   

臭氧水对番茄根结线虫病的防治效果     

胡安东  梁晨  于维霞  孙先涛  王学亮  赵洪海 《蔬菜》2019,(11):55-59

试验采用盆栽定量接种法和温室分区处理试验测定了番茄定植前臭氧水浇灌处理对南方根结线虫的防治效果。室内盆栽试验结果表明:随着臭氧水浇灌处理次数的增加,根结线虫防治效果和番茄生长促进作用呈上升趋势;与对照相比,0.5 mg/kg臭氧水浇灌处理5次对根结线虫的相对防效较好,为65.1%,番茄株高和地上部鲜质量增长率分别为60.2%和108.2%。温室分区处理试验结果表明:1.0mg/kg臭氧水浇灌处理4次,对番茄根结线虫病的相对防效为72.1%,达到熏蒸性杀线虫剂异硫氰酸甲酯相对防效的90%。  相似文献   

Partial substitution of chemical fertilizers with organic amendments increased rice yield by changing phosphorus fractions and improving phosphatase activities in fluvo-aquic soil     

Qaswar  Muhammad  Chai  Rushan  Ahmed  Waqas  Jing  Huang  Han  Tianfu  Liu  Kailou  Ye  Xinxin  Xu  Yongmei  Anthonio  Christian Kofi  Zhang  Huimin 《Journal of Soils and Sediments》2020,20(3):1285-1296

Journal of Soils and Sediments - The aim of this study was to investigate the effect of combined use of chemical fertilizers with wheat straw and swine manure on rice yield and phosphorus...  相似文献   

孔隙型储层建设地下储气库库址优选     

阳小平  程林松  郑贤斌  何学良  刘翀 《油气储运》2012,31(8):583-586,648,649

天然气地下储气库库址是关系储气库能否安全平稳运行的关键性因素,由于其选择具有多目标性,因此,在建设储气库前期必须对库址进行充分的技术和工程论证。基于国内已有储气库建设和运行的成功经验,从库址类型、构造圈闭、封闭性、储层物性、流体物性、储层埋深、老井情况、地理条件等方面,系统、定量地阐述了气藏、油藏和水藏等孔隙型储层构造改建为储气库库址的优选指标,对天然气地下储气库的规划、建设具有现实指导意义。  相似文献   

森林病虫害发生的原因及综合防治     

柴艳杰  潘明宇 《北京农业》2012,(18):111

分析森林病虫害发生的原因及病虫害综合防治的措施。  相似文献   

山地城市特色塑造与规划控制     

刘维彬  柴媛  彭宏业 《森林工程》2012,(6):91-93

随着我国城市的快速发展,城市特色形象问题逐渐成为人们关注的焦点。控制性详细规划作为衔接总体规划与修建性详细规划的关键环节,在塑造城市特色方面也起到举足轻重的作用。本文以腾冲县玛御谷温泉北片区控制性详细规划为例,从地形环境的分析入手,以具有山地城市特色的台地系统、道路系统和绿地系统为规划的重点,探讨控制性详细规划阶段,基于山地地形特征的城市特色塑造与规划控制。  相似文献   

大豆豆荚炭疽病有效杀菌剂的筛选与防治适期研究   总被引：1，自引：0，他引：1  

王国荣  孙志峰  陈吴健  林钗  夏国绵  楼曼庆  楼兵干 《浙江农业学报》2012,24(2):258-262

通过田间试验,筛选有效防治大豆豆荚炭疽病的杀菌剂,并对最佳防治时期进行研究。试验比较了8种杀菌剂对大豆豆荚炭疽病的防治效果,结果表明,防治该病害的有效杀菌剂以甲基托布津为首选,其次为施佳乐和好力克等。通过比较大豆始花期、盛花期和鼓粒期的防治效果,防效最好的是在始花期;在始花期、盛花期或鼓粒期的任何两个时期连防2次的防治效果显著好于只防1次的。在始花期与盛花期连续施药2次,防效超过95%。室内接种防治试验表明:当RH低于90%时,接种鼓粒期的豆荚喷与不喷甲基托布津都不发病;当RH为100%时,接种后24 h内喷甲基托布津,防效与对照不接菌无显著性差异,接种后48 h喷甲基托布津,防效显著低于对照不接菌。  相似文献   

Ventilation rates in large commercial layer hen houses with two-year continuous monitoring     

Chai L  Ni JQ  Diehl CA  Kilic I  Heber AJ  Chen Y  Cortus EL  Bogan BW  Lim TT  Ramirez-Dorronsoro JC  Chen L 《British poultry science》2012,53(1):19-31

1. Ventilation controls the indoor environment and is critical for poultry production and welfare. Ventilation is also crucial for assessing aerial pollutant emissions from the poultry industry. Published ventilation data for commercial layer houses have been limited, and are mostly based on short-term studies, mainly because monitoring airflow from large numbers of fans is technically challenging.

2. A two-year continuous ventilation monitoring trial was conducted at two commercial manure belt houses (A and B), each with 250?000 layers and 88 130-cm exhaust fans. All the fans were individually monitored with fan rotational speed sensors or vibration sensors. Differential static pressures across the house walls were also measured. Three fan performance assessment methods were applied periodically to determine fan degradations. Fan models were developed to calculate house ventilations.

3. A total of 693 and 678 complete data days, each containing >16?h of valid ventilation data, were obtained in houses A and B, respectively. The two-year mean ventilation rates of houses A and B were 2·08 and 2·10?m³?h^?1?hen^?1, corresponding to static pressures of ?36·5 and ?48·9?Pa, respectively. For monthly mean ventilation, the maximum rates were 4·87 and 5·01?m³?h^?1?hen^?1 in July 2008, and the minimum were 0·59 and 0·81?m³?h^?1?hen^?1 in February 2008, for houses A and B, respectively.

4. The two-year mean ventilation rates were similar to those from a survey in Germany and a 6-month study in Indiana, USA, but were much lower than the 8·4 and 6·2?m³?h^?1?hen^?1 from a study in Italy. The minimum monthly mean ventilation rates were similar to the data obtained in winter in Canada, but were lower than the minimum ventilation suggested in the literature. The lower static pressure in house B required more ventilation energy input. The two houses, although identical, demonstrated differences in indoor environment controls that represented potential to increase ventilation energy efficiency, and reduce carbon footprints and operational costs.  相似文献   

弗兰克氏菌nifH及nifD基因的定位     

曾王勇  崔玉海  柏学亮 《西北农林科技大学学报(自然科学版)》1992,20(1):5-10

利用限制性内切酶酶切和分子杂交等手段,对弗兰克氏菌菌株At4和Hr16的固氮基因克隆的nifH(pPC1201)、nifD(pPC1202)及nifK(pPC1203)基因为探针杂交,定位了At4和Hr16的nifH和nifD基因。同时发现,一条1.3kb的BamHI片段丢失。  相似文献   

Molecular characterization and functional analysis of Trx and Trp14 in roughskin sculpin (Trachidermus fasciatus)     

Liu  Yingying  Zhong  Jinmiao  Zhao  Lihua  Yu  Shanshan  Zha  Haidong  Chai  Yingmei  Zhu  Qian 《Fish physiology and biochemistry》2021,47(5):1369-1382

Fish Physiology and Biochemistry - Thioredoxins (Trxs) are a family of small and highly conserved proteins which play crucial roles in the maintenance and regulation of the cellular redox...  相似文献